Journal: Scientific Reports

Article Title: (Pro)renin receptor is crucial for Wnt/β-catenin-dependent genesis of pancreatic ductal adenocarcinoma

doi: 10.1038/srep08854

Figure Lengend Snippet: (a) Plasma s(P)RR levels in 20 patients with PDAC and 20 healthy matched controls. Data are mean ± SD values. The boxes encompass the twenty-fifth through seventy-fifth percentiles of results. The lines through the middle of each box represent the medians. The maximum and minimum values within 1.5 × interquartile range (IQR) are shown as whisker caps. Average values are indicated by dots in the boxes. (b) s(P)RR in conditioned medium was assayed for HPDE cells and six different PDAC cell lines. Consistent results were observed when three experiments were repeated. Loading control was determined by CBB staining.

Article Snippet: To obtain a loading control of s(P)RR expression, we used the CBB (Coomassie Brilliant Blue) staining solution (Wako, Osaka, Japan, catalog #17400553) to visualize the protein bands.

Techniques: Whisker Assay, Staining

Journal: Molecular Therapy. Nucleic Acids

Article Title: Albumin-conjugation enables improved tumor targeting of aptamers via SPECT imaging

doi: 10.1016/j.omtn.2025.102483

Figure Lengend Snippet: Synthesis and characterization of HSA-AS1411 (A) Purification of HSA-AS1411 and HSA-RS by IEX. The chromatogram at 260 nm showed complete separation of HSA-AS1411 (or HSA-RS) from the mixture. (B) SDS gel electrophoresis. (C) TBE-Urea gel electrophoresis. Coomassie staining (B) and nucleic acid staining (C) co-confirmed the conjugation and purity of HSA-AS1411 (or HSA-RS). Mal, maleimide.

Article Snippet: Fast Coomassie Brilliant Blue Staining Solution (10×) and 4′,6-diamidino-2-phenylindole (DAPI) were obtained from Elabscience (Wuhan, China).

Techniques: Purification, SDS-Gel, Electrophoresis, Nucleic Acid Electrophoresis, Staining, Conjugation Assay